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. 2021 Oct 21;106(3):503–514. doi: 10.1093/biolre/ioab193

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© The Author(s) 2021. Published by Oxford University Press on behalf of Society for the Study of Reproduction.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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FRZB interacts with WNT3A in granulosa cells. (A) Immunocytochemistry of granulosa cells isolated from a post-natal day (PND) 4 mouse ovary. Frzb (red) co-localized with Wnt pathway activator WNT3A. Insets show zoomed image of boxed area. (B) Duolink proximity ligation assay of granulosa cells isolated from PND4 mouse ovary, red dot indicates proteins are interacting; representative images are indicative of n = 3 biological replicates performed in triplicate. Images taken at 40× magnification. Scale bar represents 50 μm.