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. 2022 Mar 7;13:803380. doi: 10.3389/fimmu.2022.803380

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Copyright © 2022 Johnson, Zale, Frary and Lomakin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Ten-day expansion of NK cells from PBMCs with NK Cloudz and GMP human platelet lysate cultured in the G-Rex6M. (A) Flow cytometer density plots at 0, 7, and 10 days compare CD3 and CD56 expression of the expanded cell population. Black arrows indicate CD3⁻CD56⁺ (NK) cells, which correspond with the green boxes in (B) the plot of cell distribution of viable CD45⁺ cells. (C) Viable NK cell fold change relative to day 0. (D) The viability of two populations: the light gray bars represent the viability of all CD45⁺ cells (including NK cells) and the dark gray bars represent the viability of only the CD45⁺CD3⁻CD56⁺ (NK) population. The graphs are broken out by donor (Dn A, Dn B, Dn C, and Dn D) to highlight the donor variability in the process.