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. 2022 Mar 7;13:803380. doi: 10.3389/fimmu.2022.803380

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Copyright © 2022 Johnson, Zale, Frary and Lomakin

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of the xeno-free 10% GMP human platelet lysate/NK Cloudz protocol to the existing FBS/NK Cloudz protocol in the G-Rex6M. The results show (A) the cell distribution on day 10, (B) NK (CD3⁻CD56⁺) fold change between days 0 and 10, (C) the viability of either all CD45⁺ cells or NK cells alone on day 10, and (D) the percent of target K562 cells killed on day 10 with a 1:1 effector:target (E:T) ratio and a 4 h incubation. Data represent the mean ± SD from 4 separate donors. The GMP human platelet lysate resulted in similar purity, expansion, viability, and cytotoxicity as the FBS protocol.