Figure 4.

Release of the cells and extended culture. (A) Diagram of the protocol used to test how extended periods in the release buffer affect cells. Samples were mixed, and then an equal volume of 1× release buffer was added to the cells to release the Cloudz. Samples were incubated in this release buffer for either 30, 60, or 120 min, then washed, returned to growth media, and cultured for 3 days. (B) The viability of either all CD45⁺ cells (light gray bars) or NK cells alone (dark gray bars). Data represent the mean ± SD from 3 separate donors. The results suggest that extended incubation has no effect on viability compared with a sample that was not released.