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. 2022 Mar 7;13:783254. doi: 10.3389/fimmu.2022.783254

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Copyright © 2022 Zhang, Tao, Chen, Zhao, Feng, Chen and Fu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Systemic and local BAFF expression in DSS-induced colitis mice and distribution in intestinal mucosa. To induce chronic colitis, mice were challenged with 2.5% DSS; 5 days of 2.5% DSS, 2 days of sterile water, four cycles. Mice were injected intraperitoneally with BAFF monoclonal antibody (2 mg/kg) on days 1 and 15, while mice in the model group were injected intraperitoneally with IgG1 negative isotype control antibody (2 mg/kg) on days 1 and 15 throughout the modeling period. Blood samples and colon were collected at the indicated time points. (A, B) Samples were assayed for BAFF concentrations by ELISA. (C) Immunofluorescence of BAFF and DAPI staining in the colon tissues (n = 6–9 per group). (D) Dual-colored immunofluorescence staining was used to determine the expression of F4/80 (specific for monocytes/macrophages) (green fluorescence) and the expression of BAFF (red fluorescence). Ctrl, control.