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LPS synergizes with BAFF to promote RAW264.7 cells inflammatory cytokines secretion. RAW264.7 cells were primed with LPS (1 ug/ml) for 6 h. Anti-BAFF antibody or mouse immunoglobulin G1 (IgG1) isotype control antibody at 1 ug/ml, recombinant mouse BAFF at 250 ng/ml in the presence of LPS 1 ug/ml for 6 h. (A–D) The mRNA level of IL-1β, TNF-α, IL-6, and IL-18 was measured by RT-qPCR. ns, P > 0.05.
