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. 2022 Mar 7;13:783254. doi: 10.3389/fimmu.2022.783254

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Copyright © 2022 Zhang, Tao, Chen, Zhao, Feng, Chen and Fu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-BAFF antibody plays an anti-inflammatory role in RAW 264.7 cell and BMDM. (A) RAW 264.7 cells were intervened with mouse anti-BAFF antibody or mouse immunoglobulin G1 (IgG1) isotype control antibody at 500 ng/ml or 1 ug/ml in the presence of LPS 1 ug/ml for 6 (h) The mRNA level of IL-1β, TNF-α, and IL-6 was measured by RT-qPCR, IL-18 was measured in cell supernatant by ELISA. (B) Bone Marrow Derived Macrophages were obtained and were treated with mouse anti-BAFF antibody at 500 ng/ml or 1 ug/ml in the presence of LPS 1 ug/ml for 6 h, pulsed with ATP (2 mM, Sigma Aldrich, USA) for 30 min. The mRNA level of IL-1β, TNF-α and IL-6 was measured by RT-qPCR, IL-18 was measured in cell supernatant by ELISA. ns, P > 0.05.