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. 2022 Mar 7;9:815916. doi: 10.3389/fcvm.2022.815916

FIGURE 3.

FIGURE 3

PAK2 attenuated DOX-induced cardiomyocyte apoptosis. (A) H9c2 was transfected with PAK2-overexpressing plasmid for 24 h. The expression levels of PAK2 were detected by Western blot and the quantitative histogram was shown. (B) H9c2 was transfected with PAK2 small-interfering RNA (siRNA) for 24 h. The expression levels of PAK2 were detected by Western blot and the quantitative histogram was shown. (C–E) Enhanced PAK2 expression with PAK2-overexpressing plasmid for 24 h and exposed to 2 μM DOX for 24 h. Apoptosis was detected by TUNEL assay (C). Green, TUNEL-positive nuclei; blue, DAPI-stained nuclei; scale bar, 200 μm. Statistical analysis of TUNEL-positive cells (D) and caspase-3/7 activity (E) are shown. (F) H9c2 was cotransfected with miR-194-5p mimic and PAK2-overexpressing plasmid for 24 h and then exposed to 0.2 μM DOX for 24 h. Cell death rate was analyzed. (G) H9c2 was transfected with PAK2 siRNA for 24 h and exposed to 0.2 μM DOX for 24 h. Cell death rate was analyzed. All the experiments have been performed independently in triplicate, and the data were expressed as mean ± SD. *p < 0.01 as indicated.