Fig. 1.

miR-93-5p downregulatesCCND2 by binding 3′UTR of CCND2 mRNA. A TargetScan, miRGate, miRWalk were used to screen target gene of miR-93-5p. B Prediction of target binding sites between miR-93-5p and CCND2 by using database RNA22v2. C CCND2 protein level in ovarian cancer cell A2780 and ovary immortalized cell IOSE80 was measured by western blot. Tubulin was used as internal control. D The qPCR assay was performed to detect the mRNA expression of CCND2 after miR-93-5p mimic or miR-NC transfection. Tubulin were used as internal control. **P < 0.01. E Western Blot was used to detect the CCND2 protein level after transfection of miR-93-5p mimic or miR-NC, **P < 0.01. F The luciferase activity that had been fused with wild type or mutant 3′UTR of CCND2 were tested by luciferase reporter assay, *P < 0.05