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. 2022 Jan 13;9(2):998–1007. doi: 10.1002/ehf2.13725

Figure 3.

Figure 3

Circ_0001206 regulates CRKL through acting as a ceRNA to target miR‐665. (A) starBase and Circinteractome databases predicted the mutual miRNAs binding with circ_0001206 and CRKL. (B). RIP assay confirmed the coexistence of circ_0001206, miR‐665, and CRKL in RISC. (C) RNA pull‐down assay verified the correlation between circ_0001206 and miR‐665, or CRKL and miR‐665 in H9C2 cells. (D) The putative binding sites between circ_0001206/CRKL and miR‐665 were predicted via starBase. (E) The overexpression efficacy of miR‐665 mimics was tested by RT‐qPCR in H/R‐induced H9C2 cells. (F) Luciferase reporter assays assessed the luciferase activity of circ_0001206 WT/Mut and CRKL 3′UTR WT/Mut when miR‐665 was overexpressed. **P < 0.01.