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. 2022 Jan 13;9(2):998–1007. doi: 10.1002/ehf2.13725

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© 2021 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of European Society of Cardiology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Circ_0001206 affects hypoxia/reoxygenation (H/R)‐induced cardiomyocyte injury myocardial infarction (MI) thorough targeting miR‐665 to modulate CRKL expression. (A). quantitative real‐time polymerase chain reaction (RT‐qPCR) detected the effectiveness of miR‐665 mimics in H/R‐treated H9C2 cells. (B). RT‐qPCR examined CRKL expression in H/R‐treated H9C2 cells after the transfection of pcDNA3.1, pcDNA3.1‐circ_0001206 or pcDNA3.1‐circ_0001206 + miR‐665 mimics. (C). RT‐qPCR detected the interference efficiency of sh‐CRKL#1/#2 in H/R‐treated H9C2 cells. (D). CCK‐8 assay detected the cell viability in H/R‐treated H9C2 cells after the transfection of pcDNA3.1, pcDNA3.1‐circ_0001206, pcDNA3.1‐circ_0001206 + miR‐665 mimics or pcDNA3.1‐circ_0001206 + sh‐CRKL#1. (E). Western blot examined Bax, Bcl‐3, cleaved caspase‐3 and cleaved caspase‐9 levels in H/R‐treated H9C2 cells after the transfection of pcDNA3.1, pcDNA3.1‐circ_0001206, pcDNA3.1‐circ_0001206 + miR‐665 mimics or pcDNA3.1‐circ_0001206 + sh‐CRKL#1 (n = 3). **P < 0.01.