FIGURE 6.

Phosphorylation of 14-3-3ζ shifts its localization from the cytosol to the nucleus. Endogenous expression levels of ζ (green) and pζ (magenta) were analyzed using immunofluorescence in HeLa 1.3 cells and U251 cells. Immunostaining was performed using specific primary anti-14-3-3ζ and anti-14-3-3ζ pS58 antibodies, and corresponding AF-conjugated secondary antibodies. Nuclei were stained with DAPI (blue). Scale bars represent 10 µm. Negative controls were performed to exclude nonspecific binding of primary antibodies (Supplementary Figure S6).