TABLE 3.
Thermodynamic parameters of 14-3-3ζ variants. Melting temperatures and enthalpies were measured in triplicates. ΔHvH is expressed per mole of the cooperative unit corresponding to the most populated oligomeric state of the 14-3-3ζ variant, i. e. dimer for ζ and ζ_S58E or monomer for ζ_S58D, ζ_S57D_S58D, ζm and pζ.
| 14-3-3 | Tm (°C) nanoDSF | Tm (°C) DSC | ΔHcal (kcal/mol) | ΔHvH (kcal/mol) |
|---|---|---|---|---|
| ζ | 60.35 ± 0.15 | 60.28 ± 0.01 | 105.33 ± 0.58 | 195.50 ± 0.87 |
| ζ_S58E | 58.19 ± 0.09 | 57.64 ± 0.01 | 118.33 ± 0.58 | 172.17 ± 0.29 |
| ζ_S58D | 54.21 ± 0.03 | 53.89 ± 0.01 | 106.00 ± 1.00 | 206.00 ± 1.00 |
| ζ_S57D_S58D | 53.58 ± 0.10 | 53.70 ± 0.01 | 101.33 ± 0.58 | 184.33 ± 0.58 |
| ζm | 53.04 ± 0.12 | 53.34 ± 0.02 | 80.47 ± 3.59 | 204.00 ± 5.00 |
| pζ | 50.70 ± 0.11 | 50.88 ± 0.02 | 74.83 ± 0.55 | 140.67 ± 0.58 |