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The tracking pathways of single cells under cyclic stretching (St.) and non-stretching (N.St.). The contours show 30 cell traces for each substrate. The MSD was calculated from the end-to-end distance of migration traces every 1 h. The diffusivity D, obtained from the calculation of MSD, was independent upon the time interval. The directionality was characterized based on the calculation of X- and Y- components of MSD.
