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Purification of C. glabrata sterol Δ22-desaturase. SDS-PAGE analysis of the purified sterol Δ22-desaturase. Protein (2.0 μg) was analyzed on a 10% polyacrylamide–SDS gel and visualized with Coomassie blue. The molecular mass of the cytochrome P-450 was determined by comparison with standard protein molecular mass markers.
