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Purification of cytochrome P-450 from C. glabrata L5UD61a
| Fraction | P-450 concn (nmol) | Protein concn (mg) | Specific P-450 content (nmol/mg of protein) | % Recovery |
|---|---|---|---|---|
| Microsomes | 80.0 | 350.0 | 0.23 | 100 |
| Amino-octyl Sepharose | 37.0 | 4.86 | 7.6 | 46.0 |
| Hydroxyapatite | 12.0 | 0.87 | 13.8 | 15.0 |
Shown are data from a typical experiment for the purification of cytochrome P-450 from C. glabrata L5UD61, containing a gene disruption in cyp51.
