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. Author manuscript; available in PMC: 2022 Sep 8.
Published in final edited form as: Nat Struct Mol Biol. 2022 Mar 8;29(3):250–260. doi: 10.1038/s41594-022-00734-6

Extended Data Fig. 4 |. Cryo-EM of nsp13-RTC.

Extended Data Fig. 4 |

A. Gold-standard Fourier-shell correlation (FSC) plot for nsp131-RTC (yellow line), nsp132-RTC (red line), and (nsp132-RTC)2 (blue line) calculated by comparing the independently determined half-maps from cryoSPARC. The dotted line represents the 0.143 FSC cutoff which indicates a nominal resolution of 3.2 Å (nsp132-RTC), 2.9 Å (nsp132-RTC), and 3.3 Å [(nsp132-RTC)2].

B - D. The cryo-EM maps filtered by local resolution 3 are shown. The view on the right is a cross-section.

(top) Colored by subunit (key above).

(bottom) Colored by local resolution (key on the bottom).

B. Nsp131-RTC (map2).

C. Nsp132-RTC (map3).

D. (nsp132-RTC)2 (map4).

E - G. Directional 3D Fourier shell correlation calculated by 3DFSC (top) 4 and particle orientation distribution calculated by cryoSPARC (bottom).

E. Nsp131-RTC.

F. Nsp132-RTC.

G. (nsp132-RTC)2.

H. Gold-standard FSC plots for local-refined maps (dashed lines) and nsp132-RTC composite map (map9) (solid line).

I. The nsp132-RTC composite map (map9) filtered by local resolution 3.

(left) Colored by subunit.

(right) Colore by local resolution (key at the bottom).