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. Author manuscript; available in PMC: 2022 Sep 8.
Published in final edited form as: Nat Struct Mol Biol. 2022 Mar 8;29(3):250–260. doi: 10.1038/s41594-022-00734-6

Extended Data Fig. 8 |. Classification pipeline for the nsp132-BTC dataset and comparison of nsp132-RTC and nsp132-BTC class distribution.

Extended Data Fig. 8 |

A. Focused classification pipeline for nsp132-BTC dataset 6.

B. (top-middle) View of the nsp132-BTC-class4. Proteins are shown as backbone ribbons except nsp13F is shown as a transparent surface. The RNA is shown as spheres. The boxed region is magnified in the views below.

(bottom) Magnified views of the nsp13T RNA binding channel for nsp132-BTC-class4 (left) and nsp132-BTC-class1 (right). The downstream single-stranded t-RNA is shown as sticks. Cryo-EM difference densities for the downstream single-stranded t-RNA are shown (green mesh).

C. Histogram illustrating the nsp132-RTC particle distribution for the nsp132-RTC (black bars) and nsp132-BTC (white bars).