Figure 1.

TET1s deletion exacerbates atherosclerotic lesions in high-fat-diet ApoE-/- mice. (A) Schematic of TET1-FL and TET1s (genes and proteins). (B) RT-qPCR was used to measure the Tet1-FL and Tet1s mRNA levels in aortic ECs from ApoE^-/- TET1^-/-, ApoE^-/-TET1^cs/cs and ApoE^-/- mice (n=3 per group). (C-G) ApoE^-/- TET1^-/-, ApoE^-/-TET1^cs/cs and ApoE^-/- mice (8 weeks old) were fed a high-fat diet for 4 and 12 weeks, respectively. (C-D) The aortic plaques of ApoE^-/- TET1^-/- and ApoE^-/-TET1^cs/cs mice were tested by ORO staining and en face microscopy. (E) The model of whole aortas divided into aortic arch, thoracic aorta and abdominal aorta. (F-G) The lesion areas in the whole aorta, aortic arch, thoracic aorta, and abdominal aorta sections were analyzed (n>7 per group).