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. 2022 Feb 21;18(5):1912–1932. doi: 10.7150/ijbs.68484

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C16:0 acts synergistically with 5-FU to inhibit the growth and invasion induced by CAFs. A, DLD1 cells were treated with the indicated concentration of C16:0/5-FU for 48 h and assessed by annexin-V/PI assay. n=3. B, Animals from each test group were bioimaged (Xenogen IVIS system) by detecting the luciferase emission spectrum at 2 weeks after i.p. injection to visualize the progression of tumor growth in the peritoneum. n=4. C, Luciferase intensity of each test group was analyzed. Bars, mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001. D, Proposed hypothesis of the prevention of colorectal peritoneal metastasis by palmitic acid (C16:0).