Figure 6.

NR treatment extended the survival of newborn neurons in the brain of ALS SOD1G93A mice. Representative photomicrographs showed the immunostaining of DCX (A maker of newborn neuron; red) and DAPI (blue) in SVZ (A), SGZ (B), OB (C) regions at 120 days from four groups mice. Cell nuclei were counterstained with DAPI. Yellow arrows indicated the colocalization of DCX+ cell and DAPI. Bar graphs showed the quantitative analysis of DCX+ cells in SVZ (D), SGZ (E), OB (F) regions. Scale bar: 40μm. Data were expressed as mean ± SEM of n=3 mice/group; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.