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. 2022 Feb 21;18(5):1878–1895. doi: 10.7150/ijbs.69934

Figure 4.

Figure 4

MiR-197-3p targeting HSPA5 mediates the effects of NPC radioresistance. (A, B) The radioresistance of transfected cells to 4Gy radiation was observed by colony formation assay. (C, D, E) Flow cytometry was used to detect transfected cell apoptosis after 4Gy. (F) LV-has-miR-197-3p infected HK-1R cells. (G) QRT-PCR detected miR-197-3p expression in HK-1R-LV-miR-197-3p group. (H, I, J) Animal experiments have confirmed that miR-197-3p could affect the radiosensitivity of NPC. Tumor volume and weight were monitored. K. The protein levels of HSPA5 in transferred NPC cells were detected. (L, M) Dual luciferase reporter gene assay verified that miR-197-3p could directly target HSPA5. (N, O, P) Colony formation experiments confirmed the regulation of radioresistance of NPC by miR-197-3p targeting HSPA5.