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. 2022 Feb 21;18(5):1878–1895. doi: 10.7150/ijbs.69934

Figure 7.

Figure 7

Exosomal transfer of miR-197-3p inhibits NPC progression by AKT/mTOR pathway. (A) Detection of NPC-EXOs by transmission electron microscopy. (B) Western Blot confirmed the exosome-related proteins (TSG101 and HSP70). (C) QRT-PCR was compared the exosomal miR-197-3p expression. (D) HK-1 with EGFP-labeled LV-miR-197-3p. (E) QRT-PCR tested the infection efficiency. (F) The expression of miR-197-3p in LV-HK-1-EXOs was detected by qRR-PCR. (G) PKH26 - labeled exosomes were ingested by radioresistant NPC. (H) QRT-PCR to detect changes in miR-197-3p levels after EXO-miR-197-3p and EXO-CON uptake in radioresistant NPC cells. (I) The AKT/mTOR pathway changes after exosomes ingested were detected by Western Blot. (J) The expression of miR-197-3p was detected by qRT-PCR after the incubation of EXO-HK-1. (K, L) CCK-8 assay was detected the proliferation after the incubation of EXO-HK-1. (M, N) The effect of EXO-miR-197-3p on migration was examined by Wound Healing assay.