Figure 2.

The effect of SENP1 on EMT pathway in TNBC cells. The MDA-MB-231 cells was transfected with shRNA-NC or shRNA-SENP1 by lentiviral vector. SENP1 in MDA-MB-231 cells were stably knockdown by lentiviral vector. A The mRNA levels of SENP1 in BT549 cells, HCC1143 cells, HCC1937 cells, MDA-MB-231 cells was detected by qPCR. B The morphology of the MDA-MB-231cells was observed with a light microscope. C, D, E The efficiency of SENP1 knockdown were measured by WB and qPCR. F, G MDA-MB-231 cells were seeded on chambers for 24 h. Cells that migrated through the matrigel-coated chambers were stained with crystal violet. Representative images were captured, and the cells were counted from three independent experiments. H, I The lateral migration ability was measured by wound healing assays. Representative images of wound were captured at 0 h and 72 h, the healed rate is presented. J Western blot analysis of EMT pathway-related proteins as E-cadherin (K), Krt12 (L), Vimentin (M), Survivin (N), Snail (O), Slug (P), Twist (Q), ZEB1 (R), ZEB2 (S) in different group. The Results were shown as mean ± SD.*P < 0.05, **P < 0.01.