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. 2022 Mar 6;18(5):2186–2201. doi: 10.7150/ijbs.60594

Figure 5.

Figure 5

GATA1 K137R mutants impaired sumoylation of GATA1. A The MDA-MB-231 cell extracts from the were subjected to immunoprecipitation with anti-GATA1 antibody or anti-IgG antibody, followed by immunoblot with anti-SENP1 antibody. The MDA-MB-231 cells were transfected with Flag-GATA1-WT plasmid or Flag-GATA1- K137R plasmid by lentiviral vector. B, C The expression of GATA1 were detected by western blot assay. ShRNA-NC-MDA-MB-231 cells, shRNA-SENP1-MDA-MB-231 cells were transfected with Flag-GATA1-WT plasmid or Flag-GATA1- K137R plasmid by lentiviral vector. D And the cell extracts from the indicated groups were subjected to immunoprecipitation with anti-GATA1 antibody or anti-IgG antibody, followed by immunoblot with anti-SUMO1 antibody. E The promotor activity of CSN5 was measured by luciferase reporter assay. F Binding site of GATA1 with the promoter of CSN5. Results were mean ± SD for three individual experiments which, for each condition, were performed in triplicate. * P <0.05, ** P <0.01.