Figure 7.

The effect of SENP1 on the tumorigenicity and metastatic of MDA-MB-231 cells in vivo. A The tumors of mice were excised from the indicated groups after indicated treatment. B The tumor masses for two groups of animals were compared, and each histogram represented the mean ± S.D. of 8 mice. C The mouse body weight was measured every 3 days. D The tumor volumes of the indicated groups were measured and calculated once every 3 days. E The lung weight. F Representative HE staining of lungs separated from the nude mice which lung metastasis model. and each histogram represented the mean ± S.D. of 6 mice. G Number of metastatic nodes per lung was been counted. H The tubecomors tissue extracts from the indicated groups were subjected to immunoprecipitation with anti-GATA1 antibody or anti-IgG antibody, followed by immunoblot with anti-SUMO1 antibody. I, K IHC staining of tumor tissue from the indicated groups were detected by ZEB1 antibody. J, L IHC staining of tumor tissue from the indicated groups were detected by CSN5 antibody. Results were shown as mean ± SD. * P <0.05 ** P <0.01.