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. 2022 Feb 28;18(5):2132–2145. doi: 10.7150/ijbs.69325

Figure 6.

Figure 6

RNF26 promotes the degradation of CBX7 in renal cancer cells. A, A498 and 786-O cells were infected with indicated shRNAs for 72 h. After treated with or without MG132 for another 8 h, cells were harvested for Western blot analysis. B, A498 and 786-O cells were transfected with indicated plasmids for 24 h. After treated with or without MG132 for another 8 h, cells were harvested for Western blot analysis. C, A498 and 786-O cells were transfected with indicated plasmids for 24 h. Cells were harvested for Western blot analysis. D, 786-O cells were infected with the indicated shRNAs. After 72 h, cells were treated with cycloheximide (CHX), and cells were collected for Western blot analysis at different time points. E, 786-O cells were transfected with the indicated plasmids. After 24 h, cells were treated with CHX, and cells were collected for Western blot analysis at different time points. F, 786-O cells were transfected with the indicated plasmids. After 24 h, cells were collected for Western blot after treatment with MG132 for 8 h. G, 786-O cells were infected with the indicated shRNAs. After 72 h, cells were collected for Western blot after treatment with MG132 for 8 h.