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. 2022 Feb 28;18(5):2132–2145. doi: 10.7150/ijbs.69325

Figure 7.

Figure 7

CBX7 is a key mediator of RNF26-induced RCC progression. A-C, A498 and 786-O cells were infected with indicated shRNAs for 72 h. Cells were collected for Western blot analysis (A), RT-qPCR (B) and CCK-8 assay (C). Data presents as mean ± SD with 3 replicates. ***, P < 0.001. D-F, A498 and 786-O cells were transfected with indicated plasmids for 24 h. Cells were collected for Western blot analysis (D), RT-qPCR (E) and CCK-8 assay (F). Data presents as mean ± SD with 3 replicates. ***, P < 0.001. G-I, A498 and 786-O cells were infected with indicated constructs for 72 h. Cells were collected for Western blot analysis (G), RT-qPCR (H) and CCK-8 assay (I). Data presents as mean ± SD with 3 replicates. ***, P < 0.001. J and K, A498 and 786-O cells were infected with indicated constructs for 72 h. Cells were collected for Western blot analysis (J) and CCK-8 assay (K). Data presents as mean ± SD with 3 replicates. Ns, not significant; *, P < 0.05; **, P < 0.01; ***, P < 0.001. L and M, A498 and 786-O cells were infected with indicated shRNAs for 72 h. Cells were collected for Western blot analysis (L) and CCK-8 assay (M). Data presents as mean ± SD with 3 replicates. Ns, not significant; *, P < 0.05; ***, P < 0.001. N-P, 786-O cells were infected with indicated constructs for 72 h. After puromycin selection, cells were subcutaneously injected into the nude mice. The image of xenografts was shown in the panel N. The tumor mass was shown in the panel O. The tumor growth curve was shown in the panel P. Data presents as mean ± SD with 5 replicates. **, P < 0.01; ***, P < 0.001.