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. 2022 Feb 21;18(5):1944–1960. doi: 10.7150/ijbs.70408

Figure 4.

Figure 4

IL-17 induces LPC transformation into CSCs through miR-122 downregulation. (A) Top 10 up (white bars) and down (blue bars) of the most dysregulated microRNAs in IL-17-treated LPC compared to untreated cells after 40 days of culture. (B) Expression of miR-122 was assessed by qRT-PCR in IL-17-treated or not (control) LPC for 20 or 40 days. (C) Control (LPC CT) or IL-17-pretreated LPC for 30 days (LPC IL-17) were transfected with either a control miRNA (mir-CT) or miR-122 mimic and (D) next cultured in sphere formation assay for 7 days (scale bar 100 μm). (E) Representative micrographs showed number of formed spheroids with a diameter greater than 50 µm (F) Messenger RNA expressions of Albumin, Aldh1A1 and cell-cycle markers (Cyclin D, E, Pcna) were assessed by qRT-PCR in IL-17 pretreated LPC for 30 days and transfected either with miR-CT or miR-122 mimic. Data represent mean ± SEM, n=6 per condition. *P < 0.05; **P < 0.01 for control versus IL-17 treated or pretreated cells; #P<0.05 and ###P < 0.001 for miR-CT treated cells versus miR-122 mimic-treated cells.