Fig. 1.

In vitro generation and characterization of allogenic HSC-engineered iNKT (^AlloHSC-iNKT) cells. a Experimental design to generate ^AlloHSC-iNKT cells in vitro. CB cord blood, HSC hematopoietic stem cell, SG suicide gene, Lenti/iNKT-SG lentiviral vector encoding an iNKT TCR gene and a suicide gene, ATO artificial thymic organoid. b Generation of iNKT cells (identified as iNKT TCR⁺CD3⁺ cells) during ATO culture. A 6B11 monoclonal antibody was used to stain iNKT TCR. c Generation of iNKT cells (identified as iNKT TCR⁺CD3⁺ cells) during Feeder-free culture. d Yields of ^AlloHSC-iNKT cells generated from ATO and Feeder-free cultures. e FACS characterization of surface marker expression and intracellular cytokine and cytotoxic molecule production of ^AlloHSC-iNKT cells. Periphery blood mononuclear cell (PBMC)-derived iNKT (PBMC-iNKT) cells and conventional αβ T (PBMC-Tc) cells were included as controls. Representative of over 5 experiments