Figure 5. PVN^OXT neuron silencing dysregulates insulin secretory apparatus.

(A) Schematic representation of YFP and TeNT virus injection site with representative images of viral YFP (n=7, 4 images per mouse) or EGFP (n=7, 4 images per mouse) expression in PVN^OXT neurons, respectively.

(B) 24h-fasted and fed plasma insulin (left) and blood glucose (right) levels in PVN^OXT:YFP (n = 7) and PVN^OXT:TeNT (n = 7) mice. Graph shows mean ± sem. *P < 0.05, ***P < 0.001, ns: non-significant; two-tailed, unpaired Student’s t-test.

(C) KEGG pathways significantly altered upon PVN^OXT silencing in islets from 24h-fasted mice (n = 4 per group).

(D) Heat map of expression patterns of significantly down- or upregulated genes implicated in granule docking (n = 4 per group).

(E) Fold change in mRNA levels of genes implicated in regulation of insulin secretion (n = 4 per group).

(F) Map of gene products involved in insulin secretion significantly altered in PVN^OXT:TeNT mice (affected genes highlighted in blue; unaffected genes in grey) (n = 4 per group).

All data from the RNASeq were selected with a cutoff at *P < 0.05.