Extended Data Table 4|.
Immunoglobulin gene usage of S-binding monoclonal antibodies
| Heavy Chain |
Light Chain |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Native isotype | Gene usage | Nucleotide mutations* | AA mutations | HCDR3 | Gene usage | Nucleotide mutations* | AA mutations | LCDR3 |
| 07.1A11 | IgM | VH3-15 DH1-7 JH4 | 4/283=0.0141 | 4 | TTGWFTGTYGDYFDY | VK1-33 JK4 | 1/267=0.0037 | 1 | QQYDNLPPT |
| 07.1A12 | IgA1 | VH3-49 D2-15 JH4 | 3/281=0.0107 | 3 | TRVKYCSGGSCYGYHFDH | VK3-15 JK3 | 4/262=0/0.0153 | 3 | QQYNNWFT |
| 07.1E10 | IgG1 | VH4-34 D6-19 JH6 | 5/272=0.0184 | 5 | ARVVIAVAGTYPIQVYYYYGMDV | VK1-27 JK1 | 2/266=0.0075 | 2 | QKYNSAPRT |
| 07.1E11 | IgA1 | VH3-30 D3-22 JH6 | 1/278=0.0036 | 1 | AKEEMIEDWGMDV | VL3-1 JL2 | 2/265=0.0075 | 2 | QAWDRSTVV |
| 07.1H09 | IgG1 | VH3-66 DH3-10 JH3 | 3/275=0.0109 | 3 | ARDFREGAFDI | VK1-9 JK4 | 0/266=0 | 0 | QQLNSYPPT |
| 07.2A07 | IgG1 | VH3-21 DH2-21 JH4 | 6/277=0.0217 | 6 | ARAGFVPKRAYCGGDCWYYFDY | VK3-11 JK4 | 0/263=0 | 0 | QQRSNWLT |
| 07.2A08 | IgG1 | VH4-4 DH6-19 JH4 | 2/275=0.0073 | 2 | ATDGGWYTFDH | VL3-1 JL2 | 3/265=0.0113 | 3 | QAWGSSTVV |
| 07.2A10 | IgG1 | VH4-31 DH3-16 JH3 | 1/278=0.0036 | 1 | ARYPVWGAFDI | VK1-33 JK3 | 3/267=0.0112 | 3 | QHYDNLPPT |
| 07.2C08 | IgG1 | VH1-58 DH2-15 JH3 | 2/275=0.0073 | 2 | AAAYCSGGSCSDGFDI | VK3-20 JK1 | 5/266=0.0188 | 5 | QQYGSSPWT |
| 07.2C12 | IgG1 | VH3-30-3 DH1-26 JH3 | 3/277=0.0108 | 3 | ARARGGSYSGAFDI | VK3-20 JK2 | 1/267=0.0037 | 1 | QQYGSSPMYT |
| 07.3D07 | IgG1 | VH3-30-3 DH5-18 JH4 | 3/277=0.0108 | 3 | ARVLWLRGMFDY | VL6-57 JL3 | 2/278=0.0072 | 1 | QSYDISNHWV |
| 07.4A07 | IgG1 | VH3-30-3 DH3-10 JH4 | 3/277=0.0108 | 2 | ARGDYYGSGSYPGKTFDY | VK1-33 JK4 | 1/266=0.0038 | 1 | QQYDNLPLT |
| 07.4B05 | IgG1 | VH1-69 DH1-26 JH5 | 1/277=0.0036 | 1 | ARGRLDSYSGSYYSWFDP | VK4-1 JK2 | 2/283=0.0071 | 2 | QQYYSTPYT |
| 07.4C10 | IgG1 | VH3-23 DH3-22 JH4 | 1/276=0.0036 | 1 | AKNEMAMIVVVITLFDY | VL1-51 JL3 | 2/277=0.0072 | 2 | GTWDRSLSAWV |
| 07.4D09 | IgG1 | VH4-4 DH2-15 JH4 | 1/274=0.0036 | 1 | ATKYCSGGSCSYFGY | VL2-23 JL3 | 0/277=0 | 0 | CSYAGSSTWV |
| 20.1A12 | IgG1 | VH3-30 DH1-26 JH4 | 2/277=0.0072 | 2 | AKGHSGSYRAPFDY | VK3-20 JK2 | 0/263=0 | 0 | QQYGSSYT |
| 20.2A03 | IgM | VH3-33 DH3-10 JH4 | 1/278=0.0036 | 1 | AREAYFGSGSSPDY | VL3-10 JL2 | 2/272=0.0074 | 2 | YSTDSSDNHRRV |
| 20.3C08 | IgG1 | VH3-7 DH3-22 JH4 | 1/278=0.0036 | 1 | AREGTYYYDSSAYYNGGLDY | VL3-10 JL2 | 2/268=0.0075 | 2 | YSTDSGGNPQGV |
| 20.3D12 | IgG1 | VH3-33 DH1-1 JH4 | 7/278=0.0252 | 7 | ATEPVQLEPEVRLDY | VL3-10 JL1 | 1/272=0.0037 | 1 | YSTDSSGNHRRL |
| 20.3F12 | IgG1 | VH3-7 DH4-11 JH4 | 0/278=0 | 0 | ARDQGVTTGPFDY | VL3-1 JL2 | 1/265=0.0038 | 1 | QAWDSSTVV |
| 22.1A11 | IgG3 | VH3-30-3 DH3-16 JH4 | 0/278=0 | 0 | ARDLVVWEELAGGY | VL3-10 JL3 | 2/270=0.0074 | 2 | YSTDSSGNHGV |
| 22.1B04 | IgG1 | VH3-30 DH2-15 JH4 | 4/279=0.0143 | 3 | AKQGGGTYCGGGSCYRGYFDY | VK1-33 JK4 | 2/266=0.0075 | 2 | QQYDNLPLT |
| 22.1B08 | IgA1 | VH1-46 DH4-17 JH3 | 16/278=0.0576 | 15 | ARDPRVPAVTNVNDAFDL | VK3-11 JK2 | 5/267=0.0187 | 5 | QQRSNRPPRWT |
| 22.1B12 | IgG1 | VH3-53 DH3-10 JH4 | 8/273=0.0293 | 5 | ARSHLEVRGVFDN | VK4-1 JK2 | 1/282=0.0035 | 1 | QQYYSTPCS |
| 22.1C02 | IgG1 | VH3-20 DH7-27 JH4 | 5/275=0.0182 | 4 | ARGTGAADY | VK3-20 JK2 | 6/266=0.0226 | 6 | QQYGRSPYT |
| 22.1E07 | IgA1 | VH3-33 DH4-17 JH4 | 3/278=0.0108 | 3 | AREGVYGDIGGAGLDY | VL3-10 JL1 | 4/272=0.0147 | 2 | YSTDSSVNGRV |
| 22.1E11 | IgG1 | VH3-30 DH2-15 JH4 | 2/274=0.0073 | 2 | AKMGGVYCSAGNCYSGRLEY | VK1-33 JK3 | 0/263=0 | 0 | QQYDNLLT |
| 22.1G10 | IgG1 | VH4-59 DH2-21 JH5 | 12/275=0.0436 | 11 | ARETVNNWVDP | VK4-1 JK1 | 10/282=0.0355 | 8 | QQYFTTPWT |
| 22.2A06 | IgG3 | VH5-51 DH3-3 JH4 | 1/277=0.0036 | 1 | ARREWGGSLGHIDY | VL6-57 JL2 | 4/276=0.0145 | 4 | QSFDSSNVV |
| 22.2A08 | IgG1 | VH4-59 DH2-2 JH6 | 4/274=0.0146 | 4 | ARGQGVPAALYGMDV | VL1-40 JL2 | 3/281=0.0107 | 3 | QSYDGSLSGSV |
| 22.2B06 | IgM | VH3-53 DH1-1 JH6 | 2/275=0.0073 | 2 | ARDLQLYGMDV | VL3-21 JL2 | 2/268=0.0075 | 2 | QVWDSSSDPVV |
| 22.2F03 | IgM | VH1-18 DH6-13 JH6 | 1/277=0.0036 | 1 | ARVPGLVGYSSSWYDNEKNYYYYYYGMDV | VL3-25 JL1 | 1/270=0.0037 | 1 | QSADSSGTYV |
| 22.3A06 | IgG1 | VH3-23 DH5-18 JH5 | 2/277=0.0072 | 2 | AKADTAMAWYNWFDP | VK3-11 JK4 | 3/264=0.0114 | 3 | QHRSNWPLT |
| 22.3A11 | IgG1 | VH4-34 DH7-27 JH2 | 1/270=0.0037 | 1 | ARVWVRWWYFDL | VL3-21 JL1 | 4/272=0.0147 | 4 | QVWDNSSDQPNYV |
| 22.3A12 | IgG1 | VH1-46 DH2-21 JH4 | 4/275=0.0145 | 4 | ASSLPARGGVPGRLNY | VL1-51 JL2 | 3/277=0.0108 | 3 | GTWDSSLSVVV |
| 22.3D11 | IgG1 | VH5-51 D4-17 JH5 | 1/278=0.0036 | 1 | ARHHLDYDDYVGHWFDP | VL1-40 JL3 | 0/281=0 | 0 | QSYDSSLSGSGV |
| 22.3F08 | IgG1 | VH1-18 DH2-2 JH6 | 4/275=0.0145 | 4 | ASCPRRPAAIGDYYGMDV | VL3-21 JL7 | 1/271=0.0037 | 1 | QVWDSSSYHAV |
*
V-region nonsynonymous nucleotide substitutions