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Kinetic parameters for turnover of penicillin G by the class C β-lactamase from E. cloacae P99
| ηrel (% viscogen) | kcat (s−1) | Km (μM) | kcat/Km (M−1 s−1) |
|---|---|---|---|
| 1 (0) | 22 ± 5 | 10 ± 3 | (2.1 ± 0.8) × 106 |
| 1.49 (13% sucrose) | 21 ± 4 | 11 ± 4 | (1.8 ± 0.7) × 106 |
| 1.93 (21.6% sucrose) | 21 ± 2 | 12 ± 3 | (1.8 ± 0.4) × 106 |
| 2.92 (28% sucrose) | 22 ± 6 | 14 ± 6 | (1.6 ± 0.8) × 106 |
