Fig. 1.

Effects of D. typus venom, and inhibition by small molecule drugs, on in vitro plasma clotting measured by absorbance at 595 nm (OD₅₉₅).A) Clotting as indicated by the increase in OD₅₉₅ in the presence of crude D. typus venom (black circles) compared to normal clotting in the absence of venom (grey squares). Data points represent the mean of twelve individual values recorded over three independent technical replicates, and error bars represent standard deviation. B) Inhibitory activity of marimastat (teal circles), prinomastat (dark purple circles), dimercaprol (black circles) and DMPS (pink circles) over a two-fold serial dilution curve, from which IC₅₀ values were calculated. Inhibitory activity is expressed as a percentage of normal clotting, where 100% inhibition represents return of clotting to normal plasma clotting levels. Data points represent the mean of six individual values recorded over three independent technical replicates, and error bars represent standard deviation. C) Calculated IC₅₀ values for marimastat, prinomastat, dimercaprol and DMPS. Data points represent the best fit IC₅₀ value and error bars represent 95% confidence intervals (not calculated for dimercaprol and DMPS).