Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 21;15:30. doi: 10.1186/s13045-022-01245-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 5 — Cytotoxic effect of α-KG in AML cells with wild-type IDH2. a Induction of apoptosis by cell-permeable DM-αKG in AML cell lines (U937 and ML-1) and primary AML cells isolated from patients with wt-IDH2 (AML#1 and AML#2). Cells were treated with the indicated concentrations of DM-αKG for 48 h, and apoptosis was measured by flow cytometry analysis of annexin-V positivity. The number inside each panel shows the percentage of dead cells. b, c Quantitation of the concentration-dependent apoptosis induced by DM-αKG in U937 and ML-1 cells (n = 3, mean ± SD). d Apoptosis of human primary AML cells harboring wild-type (n = 7) or mutant IDH2 (IDH2-R140Q, n = 2) treated with various concentrations (2, 4, 6, 8 and 10 mM) of DM-αKG for 48 h. Apoptosis was measured by flow cytometry analysis of annexin-V positivity