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. 2022 Mar 21;15:97. doi: 10.1186/s13071-022-05227-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2a–c — Dynamic expression profiles of Oncomelania hupensis AOX (OhAOX) messenger RNA (mRNA) and OhAOX protein in O. hupensis snails under wettable powder of niclosamide (WPN) stress. a Expression profiles of OhAOX in the whole snail, head and foot muscle (head-foot) region, and liver and gonad (liver-gonad) region after WPN treatment. Asterisks indicate significant difference (*P < 0.05, **P < 0.01) between time points and 0 h within the same treatment; underlined asterisks indicate significant difference between WPN- and H₂O-treated snails at the same time point. b Expression of OhAOX protein (upper panel) in the whole snail 0–48 h after WPN and H₂O treatment. The OhAOX mRNA profile calculated using quantitative real-time PCR (qPCR) is shown in the lower panel (and corresponds with the whole snail data in a). c Statistical analysis of OhAOX protein expression in the western blot (b); *P < 0.05, ***P < 0.001. bp Base pairs