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. 2022 Mar 21;15:97. doi: 10.1186/s13071-022-05227-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3a, b — Tissue expression of OhAOX mRNA in O. hupensis after WPN treatment following in situ hybridisation. a Tissue distribution and statistical evaluation of OhAOX mRNA in the muscle of the head-foot region. b Tissue distribution and statistical evaluation of OhAOX mRNA in the liver. The purple-blue area was identified as a positive signal of OhAOX mRNA. Asterisks indicate statistically significant difference (*P < 0.05, **P < 0.01) in the quantity of OhAOX mRNA between the given time point and 0 h under the same treatment. Underlined asterisks indicate the level of significant difference between WPN- and H₂O-treated groups at the same time point. IOD Integrated optical density; for other abbreviations, see Fig. 2