Table 1.
Group | 6 h | 12 h | 24 h | 48 h | χ2 | P |
---|---|---|---|---|---|---|
H2O | 5.00 ± 7.07 | 3.33 ± 2.36 | 1.67 ± 2.36 | 5.33 ± 4.11 | 0.247 | 0.684 |
Methanol | 1.67 ± 2.36 | 7.22 ± 6.14 | 2.78 ± 2.08 | 12.50 ± 2.50 | 3.761 | 0.056 |
SHAM | 6.67 ± 6.24 | 5.00 ± 4.08 | 1.67 ± 2.36 | 12.50 ± 2.50 | 0.751 | 0.415 |
0.04 mg/L WPN | 0.00 ± 0.00 | 0.00 ± 0.00 | 1.25 ± 2.17 | 10.89 ± 11.69 a, b | 14.656 | 0.000 |
0.06 mg/L WPN | 1.25 ± 2.17 | 1.25 ± 2.17 | 8.75 ± 4.15 | 32.50 ± 10.90* a, b, c | 40.815 | 0.000 |
0.08 mg/L WPN | 2.50 ± 2.50 | 2.50 ± 2.50 | 18.57 ± 7.49* a, b | 60.24 ± 13.12* a, b, c | 88.298 | 0.000 |
0.1 mg/L WPN | 5.24 ± 0.34 | 32.21 ± 12.66* a | 49.58 ± 9.60* a | 91.67 ± 8.03* a, b, c | 144.024 | 0.000 |
0.04 mg/L WPN + SHAM | 0.00 ± 0.00 | 3.67 ± 2.62 d | 34.90 ± 3.74* a, b | 91.72 ± 6.02* a, b, c | 216.473 | 0.000 |
0.06 mg/L WPN + SHAM | 2.50 ± 2.50 | 7.32 ± 4.02 d | 77.50 ± 5.59* a, b, d | 95.00 ± 3.54* a, b, c | 194.708 | 0.000 |
0.08 mg/L WPN + SHAM | 11.25 ± 4.15 | 34.29 ± 12.05* a | 82.50 ± 5.59* a, b, d | 100.00 ± 0.00* a, b, c, d | 161.253 | 0.000 |
0.1 mg/L WPN + SHAM | 19.29 ± 3.75* d | 46.68 ± 6.74* ad | 87.32 ± 9.17* a, b, d | 100.00 ± 0.00* a, b, c, d | 189.074 | 0.000 |
Lowercase letters indicate the following: a, mortality at that time point was significantly higher than that at 6 h for the same group (P < 0.05); b, mortality at that time point was significantly higher than that at 12 h for the same group (P < 0.05); c, mortality at that time point was significantly higher than that at 24 h, for the same group (P < 0.05); d, significant difference in mortality between the treatment group and the 0.1 WPN group at that time point (P < 0.05)
* P < 0.05 (significant difference in mortality between that treatment group and the H2O group at that time point)