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. 2022 Mar 21;20:68. doi: 10.1186/s12915-022-01267-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — GATD3A influences mitochondrial dynamics. A 1,2-dicarbonyl slot blot immunoreactivity pulse-chase assay and cellular morphology of glyoxal (GO)-treated Gatd3a^+/+ or Gatd3a^−/− MEFs. GAPDH is used as a loading control (p = *<0.05, two-tailed Student’s t test, n = 4, +SEM). B Oxygen consumption rate is decreased in Gatd3a^−/− mouse embryonic fibroblasts (MEFs) compared to wildtype. Treatment with methylglyoxal (0.2 mM) for 24 h prior to assay exacerbates observed phenotype in knockout cells. Data is normalized to cell number (p = *<0.05, p = *<0.01, two-tailed Student’s t test, n = 3 biological replicates, N = 4 technical replicates per plate, N = 3 replicates per genotype, error bars ±SEM). C Mitochondrial morphology is not significantly disrupted in Gatd3a^−/− MEFs (N = 21 per genotype, two-tailed Welch’s t-test, lines represent mean value, extended data in Additional file 1, Fig. S4B). D Overexpression of GATD3A in Gatd3a^+/+ MEFs leads to reduced mitochondrial content and fragmented mitochondrial network (N = 21 per condition, p = ****<0.0001, ***<0.001, two-tailed Welch’s t-test, lines represent mean value)