Table I.

Mass Spectrometer Detection Parameters

Compound	RT (min)	Precursor ion (m/z)	Product ions (m/z)	Dwell (s)	Cone (V)	Collision (V)
4ʹ-Hydroxy nitazene	1.79	369.2	100.0a	0.011	46	24
			72.0			32
Flunitazene	4.74	371.2	100.0a	0.011	60	26
			109.0			46
			72.0			34
5-Amino isotonitazene	1.21	381.2	100.0a	0.011	48	22
			72.0			42
Metonitazene	4.57	383.2	100.0a	0.011	48	22
			121.0			34
			72.0			20
N-Desethyl isotonitazene	6.45	383.2	72.0a	0.011	48	20
			311.9			18
			130.0			56
Clonitazene	5.98	387.1	100.0a	0.011	66	24
			124.9			36
			72.0			36
Etonitazene	5.76	397.2	100.0a	0.011	52	22
			106.9			52
			72.0			42
Protonitazene	6.69	411.2	100.0a	0.011	46	20
			106.9			52
			72.0			42
Isotonitazene	6.34	411.2	100.0a	0.011	46	20
			106.9			52
			72.0			42
Isotonitazene-d7	6.34	418.2	100.0a	0.011	50	24
			72.0			46
			107.9			52
Butonitazeneb	7.63	425.2	100.0a	0.011	46	20
			106.9			52
			72.0			42
Etodesnitazeneb	2.81	352.2	100.0a	0.011	52	18
			106.9			40
			72.0			42

^aQuantification ion.

^bNot included in the scope of full validation—evaluated using standard addition only.