Figure 1. Heterogenous development of antibody responses in a mild COVID-19 cohort.

A) Experimental approach. 85 subjects were recruited from a single spreading event, and PBMCs and serum samples were collected for analyses of antibody responses, T cell activation assays and single-cell RNAseq. B) ID50 levels in an authentic SARS-CoV2 neutralization assay for each subject. Infection status was evaluated based on a PCR positive test (plus signs) or a negative test / absence of test (circles), as well as serology (red indicates a positive result for IgG spike, blue indicates negative). C) Scatterplots displaying the correlations between different neutralization assays, using a pseudotyped virus (ID50_pseudo and ID80_pseudo for titers neutralizing 50% or 80% of infection) or authentic SARS-CoV2 (ID50). Diagonal plots display the density of the values. D) Boxplots displaying the distribution of the ID50_pseudo, ID80_pseudo and ID50 neutralization titer values of the subjects stratified by their sex and infection status. The boxes represent −2 standard deviation (lower portion), mean (black line), and + 2 standard deviation (upper portion). The values above each violin plot represent the median values of the distribution. Brackets indicate statistical significance using a one-sided t-test with **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 and ns=non significant. E) ID50 neutralizing titers against authentic SARS-CoV2 across male (purple) and female (green) subjects. There is no significant difference between the means of the two groups based on a two-sided t-test.