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[Preprint]. 2022 Mar 15:rs.3.rs-1416969. [Version 1] doi: 10.21203/rs.3.rs-1416969/v1

Figure 2. Multiple cytokines are associated with infection and show a sex-specific correlation to neutralizing antibodies.

Figure 2

A) Heatmap displaying the log2 effect sizes of biological (sex, age and infection status) and technical (fresh vs frozen processing) factors on cytokine responses measured as fold change after restimulation with a spike (red, left columns) or nucleoprotein (blue, right columns) peptide pool (Methods). Nonsignificant values (FDR ≥ 0.1) are indicated in gray, significant values are colored by the effect size value in log2 scale. B) Box-plots displaying the distribution of IFNγ, IL-17F, IL-2, IL-22, IL-5, and TNFα level fold changes upon spike and nucleoprotein stimulation, stratified by the infection status of the patients (red infected, blue non infected). Brackets indicate statistical significance using a one-sided non-parametric Wilcoxon test with *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 and n/s indicating no statistical significance. (C-D) Scatterplots displaying the correlation between log2 fold change values of indicated cytokines after nucleoprotein (C) or spike (D) restimulation corrected for subject age and sample type (fresh or frozen) (y- axis) and neutralization titers (ID50, x-axis) for infected male (top row, purple) and female (bottom row, green) subjects.