Skip to main content
. 2022 Mar 21;17(3):e0265773. doi: 10.1371/journal.pone.0265773

Fig 1. Construction of pCDH.αCD133-αCD3-ENG and expression of αCD133-αCD3 engager protein in Lenti-X 293T cells.

Fig 1

(A) Schematic representation of the lentiviral plasmid construct encoding red fluorescence protein (RFP) and αCD133-αCD3 engager with myc-tag. (B) RFP expression in transfected and puromycin selected Lenti-X 293T cells as determined by fluorescence microscopy, and compared to untransfected cells (Lx-UTF). (C) RFP expression in transfected cells before and after puromycin selection as examined by flow cytometry. Scale bar: 100 μm.