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. 2022 Mar 21;17(3):e0265773. doi: 10.1371/journal.pone.0265773

Fig 5. Cytotoxic effect of T cells secreting αCD133-αCD3 engager.

Fig 5

(A) The cytotoxic effects of the untransduced and transduced T cells secreting αCD133-αCD3 engager co-cultured with cholangiocytes (MMNK-1) expressing mWasabi-luciferase (wLuc), and of the untransduced and transduced T cells secreting αCD133-αCD3 engager co-cultured with CCA cells expressing both CD133 (KKU-100 and KKU-213A) and mWasabi-luciferase (wLuc). Fluorescent images of viable MMNK-1 and KKU-213A cells (green) were captured after 18 hours of co-culture. (B) The histograms show the percentages of cytotoxic effects calculated from the results of (A). (*p<0.05, **p<0.01, ***p<0.001, NS–not signficantly different).