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. 2022 Mar 21;17(3):e0265773. doi: 10.1371/journal.pone.0265773

Fig 6. Proliferation and cytokine production of T cells secreting αCD133-αCD3 engager.

Fig 6

(A) Proliferation of the untransduced or transduced T cells secreting αCD133-αCD3 engager after being co-cultured with cholangiocytes (MMNK-1) and CD133+ KKU-213A cells for 0, 3, and 7 days. (B) Levels of IFN-γ production in culture medium after the untransduced or transduced T cells secreting αCD133-αCD3 engager were co-cultured with CD133+ KKU-213A cells for 18 hours as determined by enzyme-linked immunosorbent assay (ELISA). (C) TNF-α and granzyme B intracellular cytokine staining of the untransduced and transduced T cells secreting αCD133-αCD3 engager co-cultured with either cholangiocytes (MMNK-1) or CD133-expressing KKU-213A cells after 6 hours of the co-culture. (*p<0.05, **p<0.01, ***p<0.001).