Skip to main content
. 2022 Mar 21;17(3):e0265773. doi: 10.1371/journal.pone.0265773

Fig 8. Protocol for the concept and development of T cells secreting αCD133-αCD3 bispecific T cell engager (αCD133-αCD3 ENG).

Fig 8

(A) Production of T cells secreting αCD133-αCD3 engager. Peripheral blood mononuclear cells (PBMCs) were isolated and transduced with lentiviruses carrying cDNA encoding αCD133-αCD3-ENG to obtain final products, which are a mixture of untransduced and transduced T cells. (B) The αCD133-αCD3 engager molecules are secreted from the transduced T cells, which can recognize and bind to CD133 tumor-associated antigen expressed on tumor cells. (C) The inset shows the binding of αCD133-αCD3 engager molecules to transduced T cells and untransduced (bystander) T cells, which activates T cells to kill cancer cells. (Image created with BioRender.com).