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Effect of EGCg on H. pylori viability in liquid medium. H. pylori YMA78 was cultured microaerobically in Mueller-Hinton broth supplemented with 10% heat-inactivated horse serum at 37°C with reciprocation in the presence of EGCg at concentrations of 128 (▵), 64 (●), 32 (▴), 8 (■), and 0 (○) μg/ml. Culture samples (100 μl) were taken at the times indicated, and viability was measured by the plate colony count technique.
