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. 2022 Feb 10;11:e71928. doi: 10.7554/eLife.71928

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Gene (Danio rerio) mib1 ZDB-GENE-030404–2
Gene (Danio rerio) Ryk ZDB-GENE-070209–277
Genetic reagent (Danio rerio) mib1ta52b DOI:10.1016/s1534-5807(02)00409-4 ZDB-ALT-980203–1374
Genetic reagent (Danio rerio) mib1tfi91 DOI:10.1016/s1534-5807(02)00409-4 ZDB-ALT-060208–4
Genetic reagent (Danio rerio) mib1nce2a This paper See material and methods
Genetic reagent (Danio rerio) ryknce4g This paper See material and methods
Sequence-based reagent Crispr-mib1-166 This paper See material and methods
Sequence-based reagent Crispr-ryk-59251 This paper See material and methods
Sequence-based reagent mib1 exon/ intron one splice morpholino DOI:10.1016/s1534-5807(02)00409-4 See material and methods
Sequence-based reagent ryk morpholino This paper See material and methods
Sequence-based reagent wnt5b morpholino DOI:10.1002/gene.1063. See material and methods
Recombinant DNA reagent Mib1ta52b-pCS2+ DOI:10.1016/j.jmb.2006.11.096
Recombinant DNA reagent Mib1ΔRF123-pCS2+ DOI:10.1016/j.jmb.2006.11.096
Recombinant DNA reagent Mib1-pCS2+ This paper See material and methods
Recombinant DNA reagent Mib1-ΔRF3-pCS2+ This paper See material and methods
Recombinant DNA reagent Flag-Ryk-Myc-pCS2+ DOI:10.1083/jcb.200912128
Recombinant DNA reagent Ryk-GFP-pCS2+ DOI:10.1083/jcb.200912128
Recombinant DNA reagent Ryk-pCS2+ This paper See material and methods
Recombinant DNA reagent Ryknce4g-pCS2+ This paper See materials and methods
Recombinant DNA reagent Ryk-HA-pCS2+ This paper See materials and methods
Recombinant DNA reagent Ryknce4g-HA-pCS2+ This paper See materials and methods
Recombinant DNA reagent Ryk-GFP-pCS2+ This paper See materials and methods
Recombinant DNA reagent GFP-Vangl2-pCS2+ DOI:10.1083/jcb.201111009
Recombinant DNA reagent Fz2-mCherry-pCS2+ DOI:10.1083/jcb.200912128
Recombinant DNA reagent Fz7-YFP-pCS2+ DOI:10.1083/jcb.200606017
Recombinant DNA reagent ROR1-GFP-pCS2+ This paper See materials and methods
Recombinant DNA reagent ROR2-mCherry-pCS2+ DOI:10.7554/eLife.36953
Recombinant DNA reagent GFP-Rab5c-pCS2+ This paper See materials and methods
Recombinant DNA reagent RhoA-pCS2+ DOI:10.1038/ncb2632
Recombinant DNA reagent NICD-pCS2+ PMID:10357943
Recombinant DNA reagent Histone2B-mRFP-pCS2+ DOI:10.1038/nature02796
Recombinant DNA reagent Histone2B-GFP-pCS2+ This paper See materials and methods
Recombinant DNA reagent Histone2B-tagBFP-pCS2+ This paper See materials and methods
Recombinant DNA reagent Wnt5b-pCS2+ DOI:10.1083/jcb.200912128
Sequence-based reagent mib1 qPCR primer (fwd) This paper See materials and methods
Sequence-based reagent mib1 qPCR primer (rev) This paper See materials and methods
Sequence-based reagent ryk qPCR primer (fwd) This paper See materials and methods
Sequence-based reagent ryk qPCR primer (rev) This paper See materials and methods
Sequence-based reagent 36b4 qPCR primer (fwd) This paper See materials and methods
Sequence-based reagent 36b4 qPCR primer (rev) This paper See materials and methods
Antibody Anti-HA 3F10 (rat monoclonal) Roche # 11867423001,RRID:AB_390918 (1:500)
Antibody Anti-cMyc 9E10 (mouse monoclonal) Santa Cruz # sc-40, RRID: AB_2857941 (1:500)
Software, algorithm ImageJ/Fiji https://imagej.net/software/fiji/
Software, algorithm R https://www.r-project.org/
Software, algorithm RStudio https://www.rstudio.com/