Figure 1.

Clustering of lung and lung epithelial transcriptomes from sphingolipid-deficient and rhinovirus (RV)-infected mice. Serine-palmitoyl transferase (SPT) and control (Co) littermates were intranasally inoculated with RV-A1B (5 × 10⁶ Tissue Culture Infectious Dose 50 assay [TCID₅₀]/mouse) or equal volumes of mock-infected purified cell lysates. Lungs were harvested 24 hours after infection. Using magnetic bead separation, CD45⁺ cells and CD45⁻, epithelial cellular adhesion molecule (EpCAM⁺) cells were isolated from lung single-cell suspension. RNA sequencing was performed on RNA isolated from lung tissue, lung EpCAM⁺, and lung CD45⁺ cells. (A) Principal component analysis from whole lung, EpCAM⁺, and CD45⁺ cells irrespective of RV infection. (B) Sample-to-sample distances based on global gene expression of all groups. (C) Global differential gene expression (adjusted P value < 0.05) according to tissue type. Data represent four mice per group for all groups, except those removed after not meeting quality control standards including one Co sample in the lung and EpCAM⁺ cell analyses, and one SPT + RV sample in the CD45⁺ analyses. PC = principal component.