Figure 3.

Individual gene analysis following RV infection of wild-type and SPT-deficient lungs and lung epithelial and lung immune cells. In lung, lung EpCAM⁺ cells, and lung CD45⁺ cells, normalized expression values of de novo sphingolipid genes with differential expression were compared with false discovery threshold adjusted P value of 0.05. Normalized expression values calculated using variance-stabilizing transformation. Normalized expression values of Sptssa (small subunit of SPT A) in (A) lung, (B) lung EpCAM⁺ cells, and (C) lung CD45⁺ cells. Normalized expression values of Cers2 (ceramide synthase 2) in (D) lung, (E) lung EpCAM⁺ cells, and (F) lung CD45⁺ cells. Normalized expression values of Cers6 in (G) lung, (H) lung EpCAM⁺ cells, and (I) lung CD45⁺ cells. Shown are means ± SEM of four mice per group for all groups, except those removed after not meeting quality control standards, including one Co sample in the lung and EpCAM⁺ cell analyses and one SPT + RV sample in the CD45⁺ analyses. ns = not significant. *P < 0.05, **P < 0.01, and ****P < 0.0001.